Here are some of our past and current projects.
NIH Phase I STTR Grant Awarded August 2023 to Develop the Gelbrane!
We received a Phase I STTR award from NIH to develop the Gelbrane, a precast polyacrylamide gel combined with a transfer membrane, and a transfer apparatus! Thanks again to NIH, Eugene Krentsel with XLerate Health, and Tyler Tatum and 3-Phase / Ripple Technologies!
A major error-prone step in western blotting is the construction of a “transfer sandwich”, where a membrane, blotter paper, and sponges are manually arranged around the gel following electrophoresis. Errors here often result in a completely failed experiment that is only discovered after ~2-3 days, causing sample loss, increased labor, lost time, and poor reproducibility.
There is an unmet need for a product that eliminates the need for manual transfer sandwich construction, while remaining affordable and familiar to investigators.
NIH Phase I STTR Grant Awarded September 2022 to Develop our First Two Products!
We are excited to announce that we have received a Phase I STTR award from NIH to develop the Mesowestern blot into two products! This grant is based on our Mesowestern publication, driven mainly by co-founders Cameron Zadeh and Jonah Huggins.
Thanks to NIH, and to Eugene Krentsel with XLerate Health for mentoring us through the process! We are also looking forward to working with Tyler Tatum and 3-Phase / Ripple Technologies with accounting and further proposal development!
Our first product is a 96-well pre-cast gel and the second is an innovative horizontal electrophoresis apparatus that fits these gels (renderings pictured below). The best parts are that the price points will be competitive with "standard" Western blotting, and that any scientist who has done such "standard" blotting should have no trouble with the 96-well gel as the protocols are nearly indistinguishable. These gels are loadable by standard single or multichannel micropipettes and hold typical sample sizes.
Mesowestern Blot (2022)
The Mesowestern blot is the next generation of the microwestern blot, enabling simultaneous analysis of 336 ~1 microliter samples but without the cost and technical deterrents of a piezoelectric pipetting apparatus.
