Published Article: 96-Well Polyacrylamide Gel for Electrophoresis & Western Blotting

Western blotting, a stalwart technique in the analysis of specific proteins and their post-translational modifications, has long been limited to ∼10 samples per experiment. In this article, the authors present a 96-sample Western blot that adheres to the standard 96-well plate dimensional constraints and requires minimal operational deviation from standard Western blotting protocols. The results demonstrate that both wet and semidry transfer are compatible with this gel thickness, but the real excitement lies in the increased capacity this method offers. The significant trade-offs discovered, as well as the demonstration of proof of principle, are expounded upon in this article. The authors conclude that the 96-well Western blot will increase reproducibility, efficiency (cost and time ∼8-fold), and capacity for biological characterization relative to established Western blots, sparking anticipation for its potential.

This research article is published by ACS Publications - ACS Omega 2025. To read the full article, click below:

A 96-Well Polyacrylamide Gel for Electrophoresis and Western Blotting