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ST-1

ST-1 Semi-Dry Transfer Unit

Transfer proteins in as little as 90 minutes using less buffer.

ST-1
ST-1

Key Features

Product Information

Everything you need to know

Product Details

  • Semi-dry transfer unit for rapid, efficient transfer of proteins from G-Blot precast gels to nitrocellulose or PVDF membranes
  • Optimized for use with G48 and G96 precast gels — transfer area precisely accommodates G-Blot membrane dimensions
  • Completes transfer in as little as 90 minutes, with no additional cooling device required
  • Requires significantly less buffer than traditional tank (wet) transfer methods
  • Auto shut-off when lid is opened for safe operation
  • Pair with the G-Blot 2.5× Semi-Dry Transfer Buffer (STB-1-10) for optimal results

Specifications

Transfer area
140 × 140 mm
Dimensions
85 × 175 × 40 mm
Weight
1.4 kg
Voltage limit
30 V
Power limit
30 W
Current limit
1000 mA

Recommended Starting Conditions

  • Current: 500 mA (constant)
  • 6% acrylamide gels: 90 minutes
  • 10% acrylamide gels: 120 minutes
  • Note: optimal transfer time will vary depending on protein size and target of interest

Need answers?

Frequently Asked Questions

How long are the gels stable?
Our precast gels are stable for at least 1 year from the casting date, denoted on each gel as the expiration date.
Are custom gels available?

Yes, gels can be customized with different amounts of acrylamide depending on your protein separation needs.

Please contact us for any custom requirements and we will be happy to investigate possibilities.

What power supplies are compatible with the HT-1?
The HT-1 is compatible with standard lab power supplies using banana plugs. Recommended specifications include adjustable voltage and current up to 100 V and 400 mA, and at least 75 W of power.
What transfer methods are compatible with the G-Blot?
You can use either semi-dry or wet transfer, as long as your transfer unit is the right size.
Should I use nitrocellulose or PVDF membranes for transfer?

Both membrane types are compatible with the G-Blot. The best membrane for your experiment will depend on your target proteins, detection methods, and antibodies.

How is the sensitivity compared to a traditional western blot?

The G-Blot has equivalent sensitivity to a traditional western blot (Birtwistle et al., 2025).

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