High throughput western blot for core facilities

Serve every PI's samples faster,
without adding gels

Up to 96 samples on one gel, at the antibody sensitivity your users already trust. Same antibodies, buffers, and protocols, with no new validation cycle for the facility to absorb.

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Lower cost per sample

One gel and one transfer round, not seven.

96 samples per gel

Clear more of the queue in a single run.

Peer-reviewed, NIH-funded

An answer ready when a PI asks.

Nothing new to validate

Same antibodies and protocols as today.

Every gel run for one PI is capacity the next one in the queue doesn't get

Core facilities are measured on turnaround and on cost recovery. Both come down to how many samples you can process per instrument-hour and what each data point costs to produce.

Traditional 15-well gels put a hard ceiling on both. Running 96 samples means roughly seven gels, seven runs, and six to seven separate transfer and antibody incubation rounds, each one consuming reagents and staff time.

G48 and G96 change the gel format, not the workflow. The bottleneck moves without the facility taking on a new platform, a new reagent line, or a new method to validate.

Serving a 96-sample request

1 gelVersus roughly seven standard 15-well gels

1 roundOf transfer and antibody incubation, versus six to seven

30 µLPer well, so dilute lysates do not need concentrating

ZeroNew reagents or methods for the facility to validate

How high throughput western blotting runs on one gel

A standard western blot from start to finish. The gel holds more samples; nothing else about the process changes.

Step 1: Load

Every PI's request on one gel

Load up to 96 samples, replicates, and controls at up to 30 microliters per well, using the buffers and sample prep already in use.

Step 2: Run and transfer

One run, one transfer

Run in the HT-1 horizontal tank on a standard power supply, then wet transfer to nitrocellulose or PVDF.

Step 3: Detect

Existing antibodies, existing imager

Probe with the antibodies your users already rely on and image on the facility's current system. One incubation covers all 96 samples.

Western blot core facility equipment that clears the queue

One gel instead of roughly seven for a 96-sample request, and one transfer and antibody round instead of six or seven.
Lower consumable cost per sample than running the same samples across standard 15-well gels.
No dedicated instrument to buy or maintain beyond the HT-1 tank.
Runs on the antibodies, buffers, protocols, and imaging system already in the facility, including existing vendor relationships.
Up to 30 microliters of sample per well, so dilute lysates do not have to be concentrated first.
Every sample, replicate, and control runs under identical conditions on one gel.

96 well western blot gel vs. standard precast gels

	G96	Standard 15-well precast gel	96-well alternative format
Samples per gel	Up to 96	15	96
Gels needed for 96 samples	1	About 7	1
Transfer and antibody rounds	1	6 to 7	1
Dedicated instrument	No, HT-1 tank only	No	Yes
Sample volume per well	Up to 30 µL	Standard	10 to 15 µL
Antibodies and buffers	Lab's existing	Lab's existing	Proprietary loading buffer

Western blot validation data you can hand a PI

The sensitivity and throughput data behind G48 and G96 come from NIH-funded, peer-reviewed research, not internal testing alone. When a PI asks whether the format has been validated, the answer is a publication you can hand them.

Serial dilutions of recombinant alpha tubulin were run on a traditional western blot gel and a G96 gel, transferred to the same membrane type, incubated with the same antibodies, and detected with the same chemiluminescent substrate, showing equivalent sensitivity.

Built by scientists who hit the wall

Founded by an NIH-funded lab that ran into the throughput limit in its own research and engineered the fix.

Test it on your own bench

A loaner evaluation lets the facility run its own samples and antibodies before committing budget.

G48 and G96 precast polyacrylamide gel specifications

G48: 48-well precast gel, single or multi-channel loadable.
G96: 96-well precast gel, runs up to 96 samples in about one hour.
Tris-HCl chemistry, compatible with standard buffers.
Up to 30 microliters loading volume per well.

Compatible with standard power supplies, sample types, and wet transfer systems.
Nitrocellulose and PVDF membranes both supported.
Works with standard blocking and washing buffers, antibodies, and detection chemistries.

Core facility western blot questions

Will this work with the antibodies and protocols my users already rely on?

Yes. G48 and G96 run on the same antibodies, protocols, and standard buffers already in use. There are no proprietary reagents and no new validation cycle for the facility to absorb.

Is the throughput claim independently validated?

Yes. The sensitivity and throughput data are peer-reviewed and NIH-funded, not a vendor benchmark.

We already buy reagents and imaging through another vendor. Why add one more?

This is not a vendor switch. G48 and G96 run on the antibodies, protocols, and imaging system the facility already uses. It is a change to the gel step only, and it leaves existing reagent and imaging relationships in place.

What if it does not work for a particular PI's antibody or a hard-to-detect target?

That is what the loaner evaluation is for. Run the facility's own samples and antibodies on a loaner HT-1 tank before committing budget, and generate the proof in house rather than relying on someone else's case study.

What do we need to get started?

The HT-1 tank and the gels. Everything else, including antibodies, buffers, transfer equipment, and imaging, stays as it is.

Run your own samples before you commit

Put your facility's hardest antibody on a G96 gel and see the result on your own imaging system. The evaluation uses your samples, your protocols, and your bench.

Request a loaner evaluation Get a quote for your facility

Serve every PI's samples faster,without adding gels